A new family of sugar-inducible expression vectors for Escherichia coli
Résumé
A set of 11 expression vectors was constructed, each of them harbouring a cloning cassette under the control of the promoter. Some of these vectors enable expression of foreign proteins in the cytoplasm, while others include a synthetic sequence coding for a very efficient secretion signal sequence. Other features are an fl origin of replication (in plus or minus orientation) and a promoterup mutation that enhances the already very high level of expression from these vectors. With such a versatile vector family, cloning, sequencing and sitedirected mutagenesis can be performed on the same vector, and the level of expression can be defined according to the specific constraints of a given protein. © 1991 Oxford University Press.