Skip to Main content Skip to Navigation
Journal articles

Molecular architecture of the PBP2–MreC core bacterial cell wall synthesis complex

Abstract : Bacterial cell wall biosynthesis is an essential process that requires the coordinated activity of peptidoglycan biosynthesis enzymes within multi-protein complexes involved in cell division (the “divisome”) and lateral wall growth (the “elongasome”). MreC is a structural protein that serves as a platform during wall elongation, scaffolding other essential peptidoglycan biosynthesis macromolecules, such as penicillin-binding proteins. Despite the importance of these multi-partite complexes, details of their architecture have remained elusive due to the transitory nature of their interactions. Here, we present the crystal structures of the soluble PBP2:MreC core elongasome complex from Helicobacter pylori, and of uncomplexed PBP2. PBP2 recognizes the two-winged MreC molecule upon opening of its N-terminal region, revealing a hydrophobic zipper that serves as binding platform. The PBP2:MreC interface is essential both for protein recognition in vitro and maintenance of bacterial shape and growth. This work allows visualization as to how peptidoglycan machinery proteins are scaffolded, revealing interaction regions that could be targeted by tailored inhibitors.
Document type :
Journal articles
Complete list of metadata

Cited literature [51 references]  Display  Hide  Download
Contributor : Frank Thomas <>
Submitted on : Wednesday, October 18, 2017 - 12:02:32 PM
Last modification on : Tuesday, July 27, 2021 - 9:06:03 AM
Long-term archiving on: : Friday, January 19, 2018 - 12:56:37 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Carlos Contreras-Martel, Alexandre Martins, Chantal Ecobichon, Daniel Maragno Trindade, Pierre-Jean Matteï, et al.. Molecular architecture of the PBP2–MreC core bacterial cell wall synthesis complex. Nature Communications, Nature Publishing Group, 2017, 8 (1), pp.776. ⟨10.1038/s41467-017-00783-2⟩. ⟨hal-01618613⟩



Record views


Files downloads