Lignocellulolytic capability of endophytic phyllosticta sp

Saowanee Wikee (1, 2) , P. Chumnunti (3) , A. Al Kanghae (4) , Ekachai Chukeatirote (4) , S. Lumyong (2) , Craig Faulds (1)

1 BBF - Biodiversité et Biotechnologie Fongiques
2 Department of Biology, Faculty of Science
3 Institute of Excellence in Fungal Research, School of Science
4 MFU - Mae Fah Luang University [Thaïlande]

The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class of fungi whose members span a wide spectrum of lifestyles and host interactions. The endophytic fungus Phyllosticta is one members of the Dothideomycetes, causing disease in economic crops. Phyllosticta was screened for the degradation of lignocellulosic biomass of commercial relevance, such as rice straw, rice husk, sorghum, wheat straw, miscanthus, lavender flower, and lavender straw. The highest degrading strains were identified from an initial screen and further analyzed for the secretion of lignocellulosic enzymes during growth on the different biomasses. With Phyllosticta capitalensis (MFLUCC14-0233), maximum activity of arabinase (944.18 U/ml culture), cellulase (27.10 U/ml), xylanase (10.85 U/ml), pectinase (465.47 U/ml), and laccase (35.68 U/ml) activities could be detected in the secretome during growth on lavender flowers and lavender straw. Phyllosticta capitalensis is thus an interesting new strain for the production of lignocellulosic enzymes during growth on cheap agro-industrial biomass.

Mots clés: Lignocellulytic enzyme; Lignocellulosic biomass; Dothideomycetes; Biological pretreatment; Agro-industrial residues; farm wastes; biological treatment

Mots clés: ascomycète; phyllosticta; résidu industriel; déchet agricole; enzyme lignolytique; traitement biologique; biomasse lignocellulosique; phase endophyte

Domaines: Sciences du Vivant [q-bio]

Saowanee Wikee, P. Chumnunti, A. Al Kanghae, Ekachai Chukeatirote, S. Lumyong, et al.. Lignocellulolytic capability of endophytic phyllosticta sp. Journal of Bacteriology and Mycology , 2017, 4 (2). ⟨hal-01611437⟩