Effect of rumen-protected conjugated linoleic acid on ruminal biohydrogenation and transfer of fatty acids to milk in dairy goats

Philippe Schmidely (1) , S. Ghazal (1) , Valérie Berthelot (1)

1 MoSAR - Modélisation Systémique Appliquée aux Ruminants

The objective of the study was to evaluate ruminal biohydrogenation (BH) and apparent transfer of long-chain (FA) from duodenum into milk in dairy goats fed Ca salts of palm oil (CaS, 45 g/d) or lipid-encapsulated conjugated linoleic acids (LE-CIA, 45 g/d providing 4.5 g trans-10, cis-12 C18:2/d and 4.5 g cis-9, trans-11 C18:2/d). Twelve lactating multiparous dairy Saanen or Alpine goats (65 +/- 3 days in milk) were randomly allocated to two experimental treatments (CaS vs LE-CIA) for a 3 wks experiment. The goats were fitted with a ruminal cannula and a T-type duodenal cannula for the measurement of duodenal flow by supplying 2.4 g of Cr2O3/d through the rumen cannula during 3 wks: the first one was for reaching steady-state concentration of this marker in the digestive tract, the second wk was for measuring the daily flow of faecal chrome and nutrient faecal digestibility, and the third wk was for the duodenal sampling. LE-CLA did not affect intake, duodenal flow and apparent ruminal digestibility of DM, OM, NDF and starch. Compared to CaS, LE-CIA tended to decrease total digestibility of DM and NDF whereas total digestibility of starch was not affect by dietary treatments. The duodenal flow of FA was higher than FA intake in goats fed CaS or LE-CLA. LE-CIA increased the duodenal flows of C18:2 Vans-10, cis-12, and C18:2 cis-9, trans-11, leading to a calculated 16% protection from BH for both FA. The LE-CIA treatment tended to decrease the duodenal flows of C18:2 cis-9, trans-12 and C18:2 trans-9, cis-12. The ruminal biohydrogenation of C18:1 cis-9, C18:2 cis-9, cis-12, and C18:3 cis-9, cis-12, cis-15 was not affected by the LE-CIA treatment. Average apparent transfer between the duodenum and milk fat was 22%, 44%, 36% and 22% for C18:2 trans-10, cis-12, C18:2 cis-9, trans-11 + C18:1 trans-11, C18:2 cis-9, cis-12 and C18:3 cis-9, cis-12, cis-15, respectively. Apparent transfer efficiency of long-chain FA was not affected by LE-CLA treatment.

Mots clés: Fatty acid duodenal flow; Conjugated linoleic acids; Biohydrogenation; Goat

Domaines: Sciences du Vivant [q-bio]

Philippe Schmidely, S. Ghazal, Valérie Berthelot. Effect of rumen-protected conjugated linoleic acid on ruminal biohydrogenation and transfer of fatty acids to milk in dairy goats. Livestock Science, 2017, 199, pp.7-13. ⟨10.1016/j.livsci.2017.03.005⟩. ⟨hal-01607577⟩