Gene-centromere mapping in meiotic gynogenetic European seabass

Münevver Oral (1) , Julie Colléter (2, 3) , Michaël Bekaert (1) , John B Taggart (1) , Christos Palaiokostas (1) , Brendan J. Mcandrew (1) , Marc Vandeputte (4, 3) , Béatrice Chatain (3) , Heiner Kuhl (5) , Richard Reinhardt (6) , Stefano Peruzzi , David J Penman (1)

1 Institute of Aquaculture, School of Natural Sciences
2 UMR INTREPID - Intensification raisonnée et écologique pour une pisciculture durable
3 IFREMER - Institut Français de Recherche pour l'Exploitation de la Mer
4 GABI - Génétique Animale et Biologie Intégrative
5 Leibniz-Institute of Freshwater Biology and Inland Fisheries
6 MP-GC - Max Planck-Genome-centre Cologne

Background: Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. Results: From a single meiotic gynogenetic family consisting of 79 progeny, 42 million sequencing reads (Illumina, trimmed to 148 bases) resolved 6866 unique RAD-tags. The 340 male-informative SNP markers that were identified confirmed the lack of paternal contribution. A gene-centromere map was constructed based on 804 female-informative SNPs in 24 linkage groups (2n = 48) with a total length of 1251.02 cM (initial LG assignment was based on the seabass genome assembly, dicLab v1). Chromosome arm structure could be clearly discerned from the pattern of heterozygosity in each linkage group in 18 out of 24 LGs: the other six showed anomalies that appeared to be related to issues in the genome assembly. Conclusion: Genome-wide screening enabled substantive verification of the production of the gynogenetic family used in this study. The large number of telomeric and subtelomeric markers with high heterozygosity values in the meiotic gynogenetic family indicate that such markers could be used to clearly distinguish between meiotic and mitotic gynogenetics.

Mots clés: Dicentrarchus labrax; Meiotic gynogenesis; Isogenic lines; ddRAD seq; Genetic map; Gene-Centromere map; Aquaculture

Domaines: Sciences du Vivant [q-bio]

Münevver Oral, Julie Colléter, Michaël Bekaert, John B Taggart, Christos Palaiokostas, et al.. Gene-centromere mapping in meiotic gynogenetic European seabass. BMC Genomics, 2017, 18 (1), pp.1-12. ⟨10.1186/s12864-017-3826-z⟩. ⟨hal-01606975⟩