Multidrug resistance Salmonella genomic island 1 in a Morganella morganii subsp. morganii human clinical isolate from France

Eliette Schultz (1, 2, 3) , Olivier Barraud (4) , Jean Yves Madec (2) , Marisa Haenni (2) , Axel Cloeckaert (1) , Marie-Cécile Ploy (4, 5) , Benoît Doublet (1)

1 UMR ISP - Infectiologie et Santé Publique
2 AVB - Unité Antibiorésistance et Virulence Bactériennes
3 Université de Lyon
4 RESINFIT - Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques
5 Service de Bactériologie, Virologie, Hygiène [CHU Limoges]

Salmonella genomic island 1 (SGI1) is a multidrug resistance integrative mobilizable element that harbors a great diversity of antimicrobial resistance gene clusters described in numerous Salmonella enterica serovars and also in Proteus mirabilis. A serious threat to public health was revealed in the recent description in P. mirabilis of a SGI1-derivative multidrug resistance island named PGI1 (Proteus genomic island 1) carrying extended-spectrum-β-lactamase (ESBL) and metallo-β-lactamase resistance genes, blaVEB-6 and blaNDM-1, respectively. Here, we report the first description of Salmonella genomic island 1 (SGI1) in a multidrug-resistant clinical Morganella morganii subsp. morganii strain isolated from a patient in France in 2013. Complete-genome sequencing of the strain revealed SGI1 variant SGI1-L carrying resistance genes dfrA15, floR, tetA(G), blaPSE-1 (now referred to as blaCARB-2), and sul1, conferring resistance to trimethoprim, phenicols, tetracyclines, amoxicillin, and sulfonamides, respectively. The SGI1-L variant was integrated into the usual chromosome-specific integration site at the 3′ end of the trmE gene. Beyond Salmonella enterica and Proteus mirabilis, the SGI1 integrative mobilizable element may thus also disseminate its multidrug resistance phenotype in another genus belonging to the Proteae tribe of the family Enterobacteriaceae.

Mots clés: multidrug resistance; Salmonella genomic island; integrons; integrative mobilizable element

Mots clés: proteus mirabilis; salmonella enterica; séquençage du génome

Domaines: Microbiologie et Parasitologie

Eliette Schultz, Olivier Barraud, Jean Yves Madec, Marisa Haenni, Axel Cloeckaert, et al.. Multidrug resistance Salmonella genomic island 1 in a Morganella morganii subsp. morganii human clinical isolate from France. MSphere, 2017, 2 (2), 5 p. ⟨10.1128/mSphere.00118-17⟩. ⟨hal-01606703⟩