Full genome sequencing of Corriparta virus, identifies California mosquito pool virus as a member of the Corriparta virus species

Manjunatha N Belaganahalli (1) , Sushila Maan (1, 2) , Narender S Maan (2) , Kyriaki Nomikou (1) , Marc Guimera (1) , Joe Brownlie (3) , Robert Tesh (4) , Houssam Attoui (1) , Peter P C Mertens (1)

1 The Vector-Borne Viral Diseases Programme
2 College of Veterinary Sciences
3 Department of Pathology and Infectious Diseases
4 Department of Pathology

The species Corriparta virus (CORV), within the genus Orbivirus, family Reoviridae, currently contains six virus strains: corriparta virus MRM1 (CORV-MRM1); CS0109; V654; V370; Acado virus and Jacareacanga virus. However, lack of neutralization assays, or reference genome sequence data has prevented further analysis of their intra-serogroup/species relationships and identification of individual serotypes. We report whole-genome sequence data for CORV-MRM1, which was isolated in 1960 in Australia. Comparisons of the conserved, polymerase (VP1), sub-core-shell 'T2' and core-surface 'T13' proteins encoded by genome segments 1, 2 and 8 (Seg-1, Seg-2 and Seg-8) respectively, show that this virus groups with the other mosquito borne orbiviruses. However, highest levels of nt/aa sequence identity (75.9%/91.6% in Seg-2/T2: 77.6%/91.7% in Seg-8/T13, respectively) were detected between CORV-MRM1 and California mosquito pool virus (CMPV), an orbivirus isolated in the USA in 1974, showing that they belong to the same virus species. The data presented here identify CMPV as a member of the Corriparta virus species and will facilitate identification of additional CORV isolates, diagnostic assay design and epidemiological studies.

Mots clés: virus; orbivirus

Mots clés: séquençage du génome complet

Domaines: Sciences du Vivant [q-bio]

Manjunatha N Belaganahalli, Sushila Maan, Narender S Maan, Kyriaki Nomikou, Marc Guimera, et al.. Full genome sequencing of Corriparta virus, identifies California mosquito pool virus as a member of the Corriparta virus species. PLoS ONE, 2013, 8 (8), pp.e70779. ⟨10.1371/journal.pone.0070779⟩. ⟨hal-01606561⟩