A User’s View of the Parameters Derived from the Induction Curves of Maximal Chlorophyll a Fluorescence: Perspectives for Analyzing Stress

Julie Ripoll (1, 2) , Nadia Bertin (1) , Luc Bidel (3) , Laurent Urban (2)

1 PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles
2 UMR Qualisud - Démarche intégrée pour l'obtention d'aliments de qualité
3 UMR AGAP - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales

Analysis of the fast kinetics of the induction curve of maximal fluorescence represents a relatively recent development for chlorophyll a fluorescence measurements. The parameters of the so-called JIP-test are exploited by an increasingly large community of users to assess plant stress and its consequences. We provide here evidence that these parameters are capable to distinguish between stresses of different natures or intensities, and between stressed plants of different genetic background or at different developmental stages at the time of stress. It is, however, important to keep in mind that the JIP-test is inherently limited in scope, that it is based on assumptions which are not fully validated and that precautions must be taken to ensure that measurements are meaningful. Recent advances suggest that some improvements could be implemented to increase the reliability of measurements and the pertinence of the parameters calculated. We moreover advocate for using the JIP-test in combination with other techniques to build comprehensive pictures of plant responses to stress.

Mots clés: chlorophyll a fluorescence; JIP-test; potential and limits; Solanum lycopersicum L.; stress response; water deficit; kinetic energy; Solanum lycopersicum L

Mots clés: méthode d'induction; solanum lycopersicum; fluorescence chlorophyllienne; réponse au stress; déficit hydrique; stress de la plante; énergie cinétique; analyse cinétique

Domaines: Sciences du Vivant [q-bio]

Julie Ripoll, Nadia Bertin, Luc Bidel, Laurent Urban. A User’s View of the Parameters Derived from the Induction Curves of Maximal Chlorophyll a Fluorescence: Perspectives for Analyzing Stress. Frontiers in Plant Science, 2016, 7, ⟨10.3389/fpls.2016.01679⟩. ⟨hal-01605733⟩