Skip to Main content Skip to Navigation
Journal articles

Iron Homeostasis Regulates the Genotoxicity of Escherichia coli That Produces Colibactin

Abstract : The genotoxin colibactin is a secondary metabolite produced by a variety of pathogenic enterobacteria. Its biosynthesis requires the enzymatic activity of the phosphopantetheinyl transferase (PPTase) ClbA. We previously showed that ClbA can also contribute to the production of siderophores. Because the biosynthesis of siderophores is regulated by iron availability, we hypothesized that iron could also modulate the production of colibactin through the transcriptional regulation of clbA. This study revealed an increased transcription of clbA under iron-limiting conditions and a decrease of clbA expression in iron-rich media. We demonstrate that clbA transcription is regulated by both the ferric uptake regulator (Fur) and the small regulatory noncoding RNA RyhB. We evidenced that the regulation of the transcription of clbA by Fur and RyhB leads to the regulation of colibactin production. This work highlights the complex mechanism of regulation of an important virulence factor by the two major regulators of bacterial iron homeostasis, making iron a key environmental factor contributing to bacterial virulence and carcinogenesis.
Document type :
Journal articles
Complete list of metadatas

Cited literature [35 references]  Display  Hide  Download
Contributor : Archive Ouverte Prodinra <>
Submitted on : Wednesday, May 27, 2020 - 3:54:23 PM
Last modification on : Tuesday, December 8, 2020 - 10:43:45 AM


2016_Tronnet_Infection and Imm...
Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution 4.0 International License




Sophie Tronnet, Christophe Garcie, Nadine Rehm, Ulrich Dobrindt, Eric Oswald, et al.. Iron Homeostasis Regulates the Genotoxicity of Escherichia coli That Produces Colibactin. Infection and Immunity, American Society for Microbiology, 2016, 84 (12), pp.3358-3368. ⟨10.1128/IAI.00659-16⟩. ⟨hal-01605475⟩



Record views


Files downloads