Skip to Main content Skip to Navigation
Journal articles

De novo transcriptome sequencing and analysis of freshwater snail (Radix balthica) to discover genes and pathways affected by exposure to oxazepam

Abstract : Pharmaceuticals are increasingly found in aquatic ecosystems due to the non-efficiency of waste water treatment plants. Therefore, aquatic organisms are frequently exposed to a broad diversity of pharmaceuticals. Freshwater snail Radix balthica has been chosen as model to study the effects of oxazepam (psychotropic drug) on developmental stages ranging from trochophore to hatching. In order to provide a global insight of these effects, a transcriptome deep sequencing has been performed on exposed embryos. Eighteen libraries were sequenced, six libraries for three conditions: control, exposed to the lowest oxazepam concentration with a phenotypic effect (delayed hatching) (TA) and exposed to oxazepam concentration found in freshwater (TB). A total of 39,759,772 filtered raw reads were assembled into 56,435 contigs having a mean length of 1579.68 bp and mean depth of 378.96 reads. 44.91% of the contigs have at least one annotation. The differential expression analysis between the control condition and the two exposure conditions revealed 146 contigs differentially expressed of which 144 for TA and two for TB. 34.0% were annotated with biological function. There were four mainly impacted processes: two cellular signalling systems (Notch and JNK) and two biosynthesis pathways (Polyamine and Catecholamine pathways). This work reports a large-scale analysis of differentially transcribed genes of R. balthica exposed to oxazepam during egg development until hatching. In addition, these results enriched the de novo database of potential ecotoxicological models.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-01605095
Contributor : Archive Ouverte Prodinra Connect in order to contact the contributor
Submitted on : Monday, October 2, 2017 - 9:25:22 PM
Last modification on : Wednesday, May 12, 2021 - 8:12:33 AM

Licence


Copyright

Identifiers

Collections

Citation

Jean-Yves Mazzitelli, Elsa Bonnafé, Christophe Klopp, Frédéric Escudie, Florence Geret. De novo transcriptome sequencing and analysis of freshwater snail (Radix balthica) to discover genes and pathways affected by exposure to oxazepam. Ecotoxicology, Springer Verlag, 2017, 26 (1), pp.127-140. ⟨10.1007/s10646-016-1748-1⟩. ⟨hal-01605095⟩

Share

Metrics

Record views

150