Systemic Propagation of a Fluorescent Infectious Clone of a Polerovirus Following Inoculation by Agrobacteria and Aphids

Sylvaine Boissinot (1) , Elodie Pichon (2, 1) , Céline Sorin (3, 4) , Céline Piccini (4, 5) , Danièle Scheidecker (4, 5) , Ziegler-Graff Véronique (4, 5) , Véronique Brault (1)

1 SVQV - Santé de la vigne et qualité du vin
2 UMR BGPI - Biologie et Génétique des Interactions Plante-Parasite
3 IPS2 (UMR_9213 / UMR_1403) - Institut des Sciences des Plantes de Paris-Saclay
4 IBMP - Institut de Biologie Moléculaire des Plantes
5 CNRS - Centre National de la Recherche Scientifique

A fluorescent viral clone of the polerovirus Turnip yellows virus (TuYV) was engineered by introducing the Enhanced Green Fluorescent Protein (EGFP) sequence into the non-structural domain sequence of the readthrough protein, a minor capsid protein. The resulting recombinant virus, referred to as TuYV-RTGFP, was infectious in several plant species when delivered by agroinoculation and invaded efficiently non-inoculated leaves. As expected for poleroviruses, which infect only phloem cells, the fluorescence emitted by TuYV-RTGFP was restricted to the vasculature of infected plants. In addition, TuYV-RTGFP was aphid transmissible and enabled the observation of the initial sites of infection in the phloem after aphid probing in epidermal cells. The aphid-transmitted virus moved efficiently to leaves distant from the inoculation sites and importantly retained the EGFP sequence in the viral genome. This work reports on the first engineered member in the Luteoviridae family that can be visualized by fluorescence emission in systemic leaves of different plant species after agroinoculation or aphid transmission

Mots clés: polerovirus; fluorescent clone; systemic infection; aphid transmission; plant louse

Mots clés: maladie des plantes; polerovirus; bacteria; aphide; TuYV; inoculation

Domaines: Microbiologie et Parasitologie

Sylvaine Boissinot, Elodie Pichon, Céline Sorin, Céline Piccini, Danièle Scheidecker, et al.. Systemic Propagation of a Fluorescent Infectious Clone of a Polerovirus Following Inoculation by Agrobacteria and Aphids. Viruses, 2017, 9 (7), pp.166. ⟨10.3390/v9070166⟩. ⟨hal-01604201v2⟩