Host tropism and host-pathogen interplay of typhoidal Salmonella enterica

Ludovic Mallet (1) , Claire Hoede (1) , Franck Cerruti (1) , Annick Moisan (1) , Christine Gaspin (1) , Isabelle Virlogeux-Payant (2) , Inna Shomer (3) , Ohad Gal-Mor (3) , Thomas Schiex (1) , Helene Chiapello (1)

1 MIAT INRA - Unité de Mathématiques et Informatique Appliquées de Toulouse
2 UMR ISP - Infectiologie et Santé Publique
3 The Infectious Diseases Research Laboratory

The species Salmonella enterica is one of the most prevalent human and animal pathogens, it includes Non Typhoïdal Salmonella (NTS) serovars like Typhimurium and Enteridis, that are generalist pathogens with broad host specificity and Typhoïdal Salmonella (TS) serovars, like Typhi and Paratyphi A, that are specialized pathogens strictly adapted to the human host and the cause of an invasive, dangerous disease known as enteric (typhoid) fever [1,2,3]. The SalHostTrop project aims at identifying, characterizing and understanding the human-restricted tropism of Typhoidal Salmonella (TS) using comparative dual-RNAseq sequencing and other complementary approaches. We combine state of the art genome and transcriptome sequencing methods to decipher the molecular basis of host-tropism in clinical strains. We contrast the comparative genomics and differential expression analyses to explore and assess the variability and plasticity of pathogenesis routes among and between typhoidal and non-typhoidal serovars. We present our on-going work including the Pacbio long-read genomic sequencing, assembly and annotation [4] of a new S. Typhi strain (120130191) and the dual RNAseq data analysis of a pilot experiment of S. Typhimurium and S. Paratyphi A during human epithelial cells infection. The new S. Typhi strain includes one circularized complete chromosome and one plasmid of about 4.78 Mb with 4638 coding genes and 106.7 kb with 128 coding genes, respectively. The dual RNAseq pilot first analyses demonstrate the feasibility of the protocol to target both pathogen and host transcripts simultaneously during infection. We also built a S. enterica subsp. enterica reference phylogenetic tree from the super-alignment of Salmonella core genes in 214 complete genomes of various serotypes that is in agreement with previous studies and will be used to explore pseudogene content of serotypes according to their evolutionary history

Mots clés: host pathogen systems; pathogenesis

Mots clés: salmonella enterica; RNA-seq; transcriptome; interaction hôte pathogène; tropisme de l'hôte; pathogénèse; séquençage du génome

Domaines: Médecine humaine et pathologie

Ludovic Mallet, Claire Hoede, Franck Cerruti, Annick Moisan, Christine Gaspin, et al.. Host tropism and host-pathogen interplay of typhoidal Salmonella enterica. JOBIM 2017 - Journées Ouvertes en Biologie, Informatique et Mathématiques, Jul 2017, Lille, France. , 2017, Journées Ouvertes en Biologie, Informatique et Mathématiques. JOBIM 2017. ⟨hal-01603254⟩