Broncho alveolar dendritic cells and macrophages are highly similar to their interstitial counterparts

Pauline Maisonnasse (1) , Elise Bordet (1) , Edwige Bouguyon (1) , Nicolas Bertho (1)

1 VIM (UR 0892) - Unité de recherche Virologie et Immunologie Moléculaires

In human medicine, bronchoalveolar lavage is the main non-traumatic procedure allowing an insight into the respiratory Dendritic Cells (DC) and Macrophages populations. However, it has never been demonstrated in a relevant model that alveolar DC subpopulations were comparable to their interstitial counterparts. In a precedent work we observed that respiratory pig DC and Macrophages were more similar to the human ones than to the mouse ones. In the present work, thanks to our animal model, we were able to collect the rare bronchoalveolar DC and compare them to their interstitial counterparts. We observed that DC presented very similar gene-expression patterns in the alveolar and interstitial compartments, validating the study of human bronchoalveolar DC as surrogate of their interstitium counterparts.

Mots clés: interdigitating cell; monocyte-derived macrophages; interstitial cell; swine; man; gene expression

Mots clés: lavage broncho alvéolaire; cellule dendritique; macrophage; cellule interstitielle; porc; humain; expression génique

Domaines: Sciences du Vivant [q-bio]

Pauline Maisonnasse, Elise Bordet, Edwige Bouguyon, Nicolas Bertho. Broncho alveolar dendritic cells and macrophages are highly similar to their interstitial counterparts. PLoS ONE, 2016, 11 (12), ⟨10.1371/journal.pone.0167315⟩. ⟨hal-01602503⟩