DNA DAMAGE BINDING PROTEIN2 Shapes the DNA Methylation Landscape

Abstract : In eukaryotes, DNA repair pathways help to maintain genome integrity and epigenomic patterns. However, the factors at the nexus of DNA repair and chromatin modification/remodeling remain poorly characterized. Here, we uncover a previously unrecognized interplay between the DNA repair factor DNA DAMAGE BINDING PROTEIN2 (DDB2) and the DNA methylation machinery in Arabidopsis thaliana. Loss-of-function mutation in DDB2 leads to genome-wide DNA methylation alterations. Genetic and biochemical evidence indicate that at many repeat loci, DDB2 influences de novo DNA methylation by interacting with ARGONAUTE4 and by controlling the local abundance of 24-nucleotide short interfering RNAs (siRNAs). We also show that DDB2 regulates active DNA demethylation mediated by REPRESSOR OF SILENCING1 and DEMETER LIKE3. Together, these findings reveal a role for the DNA repair factor DDB2 in shaping the Arabidopsis DNA methylation landscape in the absence of applied genotoxic stress.
Type de document :
Article dans une revue
Plant Cell, American Society of Plant Biologists, 2016, 28 (9), pp.2043-2059. 〈10.1105/tpc.16.00474〉
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-01601904
Contributeur : Archive Ouverte Prodinra <>
Soumis le : lundi 2 octobre 2017 - 18:22:08
Dernière modification le : jeudi 15 novembre 2018 - 20:27:44

Lien texte intégral

Identifiants

Collections

Citation

Catherine Schalk, Stéphanie Drevensek, Amira Kramdi, Mohamed Kassam, Ikhlak Ahmed, et al.. DNA DAMAGE BINDING PROTEIN2 Shapes the DNA Methylation Landscape. Plant Cell, American Society of Plant Biologists, 2016, 28 (9), pp.2043-2059. 〈10.1105/tpc.16.00474〉. 〈hal-01601904〉

Partager

Métriques

Consultations de la notice

77