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Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks

Abstract : Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca2+ signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca2+-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of cpk10 cpk30 cpk32 triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional cpk10 cpk30 cpk32 and nlp7 mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca2+ signalling network integrates transcriptome and cellular metabolism with shoot-root coordination and developmental plasticity in shaping organ biomass and architecture.
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Submitted on : Monday, October 2, 2017 - 5:49:46 PM
Last modification on : Friday, March 27, 2020 - 3:48:18 AM

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Kun-Hsiang Liu, Yajie Niu, Mineko Konishi, Yue Wu, Hao Du, et al.. Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks. Nature, Nature Publishing Group, 2017, 545 (7654), pp.311-333. ⟨10.1038/nature22077⟩. ⟨hal-01601310⟩



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