Structure of T-DNA in plants regenerated from roots transformed by Agrobacterium rhizogenes strain A4

Lise Jouanin (1) , Philippe Guerche (1) , N. Pamboukdjian (2) , Colette Tourneur (1) , F. Casse Delbart (2) , J. Tourneur (2)

1 Laboratoire de Biologie Cellulaire
2 UMR0501, Laboratoire de Biologie cellulaire et moléculaire

the structure of the t-dna in ri-transformedplants of brassica napus, nicotiana plumbaginifolia and nicotianatabacum was analysed. all the plants studied presenta particular phenotype with wrinkled leaves. the t-dnais composed of two parts: tl and tr. the size of thetl-dna (19-20 kb) seems to be almost constant, exceptin n. tabacum where it is shorter. the tr-dna can beabsent, and its size varies from about 5-28 kb, with twopredominant lengths. the smaller size does not include theregion homologous to the tms genes of the pti t-dna.the copy number varies from one to four copies per plantgenome. tl and tr-dna are not always present in thesame copy number, but in some cases are linked together

Domaines: Sciences du Vivant [q-bio]

Lise Jouanin, Philippe Guerche, N. Pamboukdjian, Colette Tourneur, F. Casse Delbart, et al.. Structure of T-DNA in plants regenerated from roots transformed by Agrobacterium rhizogenes strain A4. Molecular and General Genetics MGG, 1987, 206 (3), pp.387-392. ⟨10.1007/BF00428876⟩. ⟨hal-01601038⟩