PURIFICATION OF GONADOTROPIN FROM RAINBOW TROUT (SALMO GAIRDNERII RICHARDSON) PITUITARY GLANDS

Bernard Breton (1) , Bernard Jalabert (1) , Pierrette Reinaud (1)

1 Station Centrale de Physiologie Animale

A method of purifying trout gonadotropic hormone is discussed. The technique consists of the following stages : alcoholic extraction, two successive gel filtrations on Sephadex G5o- and G-ioo, chromatography on DEAE-cellulose and ultrogel A¢SC.A The biological activity of fractions obtained is measured using as assay the maturation in vitro of trout oocytes. The preparation obtained has a molecular weight of about 2,7500 determined by behavior on Sephadex-G 1 00 and analytical ultracentrifugation for KS20Sw of 2.45 Polyacrylamide gel electrophoresis shows two strips of electrophoretic mobility, of 0.50 and 0.58. Analysis of the amino acid composition of the preparation indicates a number of 160 AA residues per mole. These different values are compared to the corresponding ones for c-GtH, s-GtH and mammalian gonadotropins.

Mots clés: salmonids; hormone purification; fish; rainbow trout; gonadotropin; pituitary; trout; rainbow

Mots clés: poisson; salmonidae; salmo gairdneri; truite arc en ciel; oncorhynchus mykiss; gonadotropine

Domaines: Biologie animale; Biochimie [q-bio.BM]; Biophysique

Bernard Breton, Bernard Jalabert, Pierrette Reinaud. PURIFICATION OF GONADOTROPIN FROM RAINBOW TROUT (SALMO GAIRDNERII RICHARDSON) PITUITARY GLANDS. Annales de biologie animale, biochimie, biophysique, 1976, 16 (1), pp.25-36. ⟨10.1051/rnd:19760103⟩. ⟨hal-00897014⟩