Potato cultivar identification using simple sequence repeat markers (SSR)

M. Moisan-Thiéry (1) , Sylvie Marhadour (2) , Marie-Claire Kerlan (2) , N. Dessenne (3) , M. Perramant (3) , T. Gokelaere (1) , Y. Le Hingrat (1)

1 FN3PT - Fédération Nationale des Producteurs de Plants de Pomme de Terre
2 APBV - Amélioration des Plantes et Biotechnologies Végétales
3 Comité Nord Plants de Pommes de Terre

Identification of potato cultivars is currently based on phenotypic characters. Crop inspections are needed at different stages and the increasing number of cultivars means the process is becoming more and more complex. Molecular markers are a possible complementary tool to identify potato cultivars and to rapidly check the identity of seeds lots. In this study 286 potato cultivars produced in France were characterized by Polymerase Chain Reaction (PCR) using Simple Sequence Repeats (SSR) markers. Sequential amplifications with 4 to 5 of the chosen SSR markers enabled complete discrimination between all the cultivars. The patterns were registered in a database and the procedure is now used routinely in France.

Mots clés: Genetic fingerprint; microsatellites; polymorphism; Solarium tuberosum subsp.tuberosum; potato; molecular marker

Mots clés: pomme de terre; marqueur ssr; marqueur moléculaire; identification variétale

Domaines: Sciences du Vivant [q-bio]

M. Moisan-Thiéry, Sylvie Marhadour, Marie-Claire Kerlan, N. Dessenne, M. Perramant, et al.. Potato cultivar identification using simple sequence repeat markers (SSR). Potato Research, 2005, 48 (3), pp.191-200. ⟨10.1007/BF02742376⟩. ⟨hal-01600062⟩