High throughput three-dimensional imaging of myelin fibers in the whole mouse brain

H. Rositi (1, 2) , Violaine Hubert (3) , L. Weber (2) , Ong E. (2) , L.-P. Berner (2) , C. Frindel (4) , D. Rousseau (4) , Cécile Olivier (5, 6) , M. Langer (6) , Cho T.-H. (5) , N. Nighoghossian (7) , Y. Berthezène (7) , Françoise Peyrin (5, 6) , F. Chauveau (8) , Marlène Wiart (3)

1 ISIT - Image Science for Interventional Techniques
2 CREATIS - Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé
3 CarMeN - Cardiovasculaire, métabolisme, diabétologie et nutrition
4 Images et Modèles
5 Imagerie Tomographique et Radiothérapie
6 ESRF - European Synchrotron Radiation Facility
7 MOTIVATE - Imagerie et modélisation Vasculaires, Thoraciques et Cérébrales
8 BIORAN - Radiopharmaceutical and Neurochemical Biomarkers

Injury to the white matter (WM) leads to severe functional loss in many neurological diseases such as stroke and multiple sclerosis. In-vivo neuroimaging techniques such as diffusion tensor imaging are becoming important tools for the diagnosis of WM abnormalities. However, these tools still present limitations and there is a crucial need for whole-brain ex-vivo complementary approaches to overcome them [1]. We propose a novel three dimensional (3D) imaging method to map the myelin fibers in the whole mouse brain with high throughput using in-line phase contrast X-ray tomography.

Domaines: Imagerie; Neurosciences [q-bio.NC]

H. Rositi, Violaine Hubert, L. Weber, Ong E., L.-P. Berner, et al.. High throughput three-dimensional imaging of myelin fibers in the whole mouse brain. TOPIM NEUROIMAGING - from MOLECULES to NETWORKS, Feb 2017, Les Houches, France. ⟨hal-01598092⟩