Skip to Main content Skip to Navigation
Journal articles

Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure.

Abstract : Stomatal movements are crucial for the control of plant water status and protection against pathogens. Assays on epidermal peels revealed that, similar to abscisic acid (ABA), pathogen-associated molecular pattern (PAMP) flg22 requires the AtPIP2;1 aquaporin to induce stomatal closure. Flg22 also induced an increase in osmotic water permeability (Pf) of guard cell protoplasts through activation of AtPIP2;1. The use of HyPer, a genetic probe for intracellular hydrogen peroxide (H2O2), revealed that both ABA and flg22 triggered an accumulation of H2O2 in wild-type but not pip2;1 guard cells. Pretreatment of guard cells with flg22 or ABA facilitated the influx of exogenous H2O2 Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) and open stomata 1 (OST1)/Snf1-related protein kinase 2.6 (SnRK2.6) were both necessary to flg22-induced Pf and both phosphorylated AtPIP2;1 on Ser121 in vitro. Accumulation of H2O2 and stomatal closure as induced by flg22 was restored in pip2;1 guard cells by a phosphomimetic form (Ser121Asp) but not by a phosphodeficient form (Ser121Ala) of AtPIP2;1. We propose a mechanism whereby phosphorylation of AtPIP2;1 Ser121 by BAK1 and/or OST1 is triggered in response to flg22 to activate its water and H2O2 transport activities. This work establishes a signaling role of plasma membrane aquaporins in guard cells and potentially in other cellular context involving H2O2 signaling.
Document type :
Journal articles
Complete list of metadata

Cited literature [54 references]  Display  Hide  Download
Contributor : Archive Ouverte ProdInra Connect in order to contact the contributor
Submitted on : Tuesday, May 26, 2020 - 9:28:49 AM
Last modification on : Friday, August 5, 2022 - 10:33:03 AM


RodriguesO.-et al-PNAS-2017_1....
Files produced by the author(s)


Distributed under a Creative Commons Attribution - ShareAlike 4.0 International License



Olivier Rodrigues, Ganna Reshetnyak, Alexandre Grondin, Yusuke Saijo, Nathalie Leonhardt, et al.. Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure.. Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2017, 114 (34), pp.9200-9205. ⟨10.1073/pnas.1704754114⟩. ⟨hal-01595145⟩



Record views


Files downloads