Skip to Main content Skip to Navigation
Journal articles

The Adc/Lmb System Mediates Zinc Acquisition in Streptococcus agalactiae and Contributes to Bacterial Growth and Survival

Abstract : The Lmb protein of Streptococcus agalactiae is described as an adhesin that binds laminin, a component of the human extracellular matrix. In this study, we revealed a new role for this protein in zinc uptake. We also identified two Lmb homologs, AdcA and AdcAII, redundant binding proteins that combine with the AdcCB translocon to form a zinc-ABC transporter. Expression of this transporter is controlled by the zinc concentration in the medium through the zinc-dependent regulator AdcR. Triple deletion of lmb, adcA, and adcAII, or that of the adcCB genes, impaired growth and cell separation in a zinc-restricted environment. Moreover, we found that this Adc zinc-ABC transporter promotes S. agalactiae growth and survival in some human biological fluids, suggesting that it contributes to the infection process. These results indicated that zinc has biologically vital functions in S. agalactiae and that, under the conditions tested, the Adc/Lmb transporter constitutes the main zinc acquisition system of the bacterium. A zinc transporter, composed of three redundant binding proteins (Lmb, AdcA, and AdcAII), was characterized in Streptococcus agalactiae This system was shown to be essential for bacterial growth and morphology in zinc-restricted environments, including human biological fluids.
Document type :
Journal articles
Complete list of metadata

Cited literature [38 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01594866
Contributor : Archive Ouverte Prodinra <>
Submitted on : Tuesday, September 26, 2017 - 9:20:28 PM
Last modification on : Saturday, July 3, 2021 - 12:11:34 AM
Long-term archiving on: : Wednesday, December 27, 2017 - 2:01:35 PM

File

Hiron_Mereghetti_J-Bacteriol_2...
Explicit agreement for this submission

Identifiers

Citation

Pauline Moulin, Kévin Patron, Camille Cano, Mohamed-Amine Zorgani, Emilie Camiade, et al.. The Adc/Lmb System Mediates Zinc Acquisition in Streptococcus agalactiae and Contributes to Bacterial Growth and Survival. Journal of Bacteriology, American Society for Microbiology, 2016, 198 (24), pp.3265-3277. ⟨10.1128/JB.00614-16⟩. ⟨hal-01594866⟩

Share

Metrics

Record views

210

Files downloads

366