Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical

Carlos Fernandez-De-Alba (1) , Hiroki Takahashi (1) , Alexandre Richard (1) , Yves Chenavier (2) , Lionel Dubois (2) , Vincent Maurel (1) , Daniel Lee (1) , Sabine Hediger (1) , Gaël de Paëpe (1)

1 RM - Magnetic Resonance
2 RICC - Reconnaissance Ionique et Chimie de Coordination

Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.

Mots clés: Dynamic Nuclear Polarization; Membranes; Phospholipids; NMR; Spectroscopy; Solid-State NMR Spectroscopy; Dynamic Nuclear-Polarization; Solid-State NMR; C-13-C-13 Correlation; Interdigitated Gel Phase; Signal Enhancement; Proteins; Glycerol; Bilayers; Abundance

Domaines: Physique [physics]

Carlos Fernandez-De-Alba, Hiroki Takahashi, Alexandre Richard, Yves Chenavier, Lionel Dubois, et al.. Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical. Chemistry - A European Journal, 2015, 21 (12), pp.4512-4517. ⟨10.1002/chem.201404588⟩. ⟨hal-01592879⟩