Dielectric Characterisation of Single Microalgae Cell Using Electrorotation Measurements

Yu-Sheng Lin 1 Sung Tsang 2 Rasta Ghasemi 3 Sakina Bensalem 3, 4, 5 Olivier Français 6 Filipa Lopes 5 Hsiang-Yu Wang 1 Chen-Li Sun 2 Bruno Le Pioufle 3, 4
1 NTHU - National Tsing Hua University [Hsinchu]
2 NTU - National Taiwan University [Taiwan]
3 ENS Cachan - École normale supérieure - Cachan
4 SATIE-BIOMIS - Bio-MIcroSystèmes et BioSensors
SIAME - Systèmes d'Information et d'Analyse Multi-Echelles
5 LGPM - Laboratoire de Génie des Procédés et Matériaux - EA 4038
6 ESYCOM - Electronique, Systèmes de communication et Microsystèmes
Abstract : This study demonstrates the electrorotation of single microalgae cells captured in the center of a set of planar microelectrodes within a microfluidic device, and compares the rotational speeds of single microalgae cells obtained in experiments and theoretical calculations. Negative dielectrophoresis force and rotational electric field are generated by the microelectrode set to position and rotate single microalgae cells. The rotational speed of single microalgae cells in the microfluidic device is calculated automatically using an image-processing algorithm. The electrorotation spectra of microalgae cells enduring different periods of nitrogen starvation agree with the simulation results for low-and high-lipid content cells. These results show the great potential of using electrorotation in quantifying the dielectric characteristics of microalgae cells.
Keywords : microfluidics electrorotation microalgae dielectric characteristics
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Conference papers
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ENS-CACHAN | UNIV-CERGY | SATIE | UPEC-UPEM | LGPM | IFSTTAR | CENTRALESUPELEC | SATIE-SIAME | SATIE-BIOMIS | UNIV-PARIS-SACLAY | ESYCOM | ESIEE-PARIS | UNIV-HESAM

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Yu-Sheng Lin, Sung Tsang, Rasta Ghasemi, Sakina Bensalem, Olivier Français, et al.. Dielectric Characterisation of Single Microalgae Cell Using Electrorotation Measurements. Eurosensors 2017, Sep 2017, Paris, France. pp.543 - 6, ⟨10.3390/proceedings1040543⟩. ⟨hal-01592123⟩

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