Skip to Main content Skip to Navigation
Journal articles

Correlations between effective permeability and marrow contact channels surface of vertebral endplates

Abstract : Homeostasis of the intervertebral disc relies on nutrient supply and waste clearance through the dense capillary network that is in contact with the cartilage endplate (CEP). We developed a micro-computerized tomography (micro-CT) method to quantify the marrow contact channel surface (MCCS) with the CEP and to validate the hypothesis according to which MCCS was correlated to the effective permeability of the vertebral endplate (VEP) and influenced by the mechanical stimuli. The influence of compression loading on local vascularization was investigated. Six 4-week-old skeletally immature pigs were instrumented with left pedicle screws and rod at both T5-T6 and L1-L2 levels to create asymmetrical spine tethers. After 3 months of growth, three cylindrical specimens of the VEP (one central and two lateral right and left) were obtained from both the instrumented and the control levels. We used a previously validated method for measuring permeability. Micro-CT analysis (resolution 12 mu m) yielded a gray-scale 2D-image of the discal end of each specimen converted into a binary 2D-image to derive the MCCS. Correlations between MCCS and effective permeability were assessed. Effective permeability and MCCS were significantly decreased compared to the control group especially on the tethered side (-41..5%, p = 0.004 and -52.5%, p = 0.0009, respectively). Correlations were significant and showed maximal value (r(2) = 0.430, p < 0.0001) on the tethered side involving maximal compressive loadings. Mechanical stimuli, due to unbalanced growth, altered the vascularization and the convective properties of the CEP. The cascade of mechanobiological events should offer perspectives for research on disc degeneration and attempted treatment.
Complete list of metadata

Cited literature [31 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01591232
Contributor : Open Archive Toulouse Archive Ouverte (oatao) <>
Submitted on : Monday, September 25, 2017 - 9:04:26 AM
Last modification on : Monday, June 15, 2020 - 6:21:52 PM
Long-term archiving on: : Tuesday, December 26, 2017 - 12:40:04 PM

File

laffosse_18182.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Jean-Michel Laffosse, Franck Accadbled, François Molinier, Nicolas Bonnevialle, Jérôme Sales de Gauzy, et al.. Correlations between effective permeability and marrow contact channels surface of vertebral endplates. Journal of Orthopaedic Research, Wiley, 2010, 28 (9), pp.1229-1234. ⟨10.1002/jor.21137⟩. ⟨hal-01591232⟩

Share

Metrics

Record views

216

Files downloads

281