Proposal for a unified nomenclature for target-site mutations associated with resistance to fungicides

Wesley Mair (1) , Francisco Lopez-Ruiz (2) , Gerd Stammler (3) , William Clark (4) , Fiona Burnett (5) , Derek Hollomon (6) , Hideo Ishii (7) , Tarlochan S Thind (8) , James Km Brown (9) , Bart Fraaije (10) , Hans Cools (11) , Michael Shaw (12) , Sabine Fillinger (13) , Anne-Sophie Walker (13) , Emilia Mellado (14) , Guido Schnabel (15) , Andreas Mehl (16) , Richard P Oliver (17)

1 Dept.Environm & Agri. Ctr Crop Dis Management
2 Dept Environm & Agr;Ctr Crop Dis Management
3 Ctr Agr, Fungicide Resistance Res.
4 NIAB - National Institute of Agricultural Botany
5 SRUC - Scotland's Rural College
6 Orchard house
7 Sch Agr Reg Vitalisat
8 PAU - Punjab Agricultural University
9 John Innes Centre [Norwich]
10 Rothamsted Research
11 Jealott's Hill International Research Centre, Bracknell
12 UOR - University of Reading
13 BIOGER - BIOlogie et GEstion des Risques en agriculture
14 Centro National de Microbiologia
15 Department of Agricultural and Environmental Sciences
16 Research Disease Control
17 Centre for Crop Disease Management, Department of Environment and Agriculture

Evolved resistance to fungicides is a major problem limiting our ability to control agricultural, medical and veterinary pathogens and is frequently associated with substitutions in the amino acid sequence of the target protein. The convention for describing amino acid substitutions is to cite the wild-type amino acid, the codon number and the new amino acid, using the one-letter amino acid code. It has frequently been observed that orthologous amino acid mutations have been selected in different species by fungicides from the same mode of action class, but the amino acids have different numbers. These differences in numbering arise from the different lengths of the proteins in each species. The purpose of the present paper is to propose a system for unifying the labelling of amino acids in fungicide target proteins. To do this we have produced alignments between fungicide target proteins of relevant species fitted to a well-studied archetype' species. Orthologous amino acids in all species are then assigned numerical labels' based on the position of the amino acid in the archetype protein. (c) 2016 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Mots clés: fungicide; target site; mutation; SDHI; Cyp51

Domaines: Sciences du Vivant [q-bio]

Wesley Mair, Francisco Lopez-Ruiz, Gerd Stammler, William Clark, Fiona Burnett, et al.. Proposal for a unified nomenclature for target-site mutations associated with resistance to fungicides. Pest Management Science, 2016, 72 (8), pp.1449-1459. ⟨10.1002/ps.4301⟩. ⟨hal-01588038⟩