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Simultaneous monitoring of single cell and of micro-organ activity by PEDOT:PSS covered multi-electrode arrays
Abstract : Continuous and long-term monitoring of cellular and micro-organ activity is required for new insights into
physiology and novel technologies such as Organs-on-Chip. Moreover, recent advances in stem cell technology
and especially in the field of diabetes call for non-invasive approaches in quality testing of the large quantities of
surrogate pancreatic islets to be generated. Electrical activity of such a micro-organ results in single cell action
potentials (APs) of high frequency and in low frequency changes in local field potentials (slow potentials or SPs),
reflecting coupled cell activity and overall organ physiology. Each of them is indicative of different physiological
stages in islet activation. Action potentials in islets are of small amplitude and very difficult to detect. The use of
PEDOT:PSS to coat metal electrodes is expected to reduce noise and results in a frequency-dependent change in
impedance, as shown here. Whereas detection of high-frequency APs improves, low frequency SPs are less well
detected which is, however, an acceptable trade off in view of the strong amplitude of SPs. Using a dedicated
software, recorded APs and SPs can be automatically diagnosed and analyzed. Concomitant capture of the two
signals will considerably increase the diagnostic power of monitoring islets and islet surrogates in fundamental
research as well as drug screening or the use of islets as biosensors.
https://hal.archives-ouvertes.fr/hal-01578781
Contributor : Eric Cloutet Connect in order to contact the contributor
Submitted on : Tuesday, August 29, 2017 - 5:15:11 PM
Last modification on : Monday, March 28, 2022 - 1:02:04 PM
Contributor : Eric Cloutet Connect in order to contact the contributor
Submitted on : Tuesday, August 29, 2017 - 5:15:11 PM
Last modification on : Monday, March 28, 2022 - 1:02:04 PM