Determination of a new index of sexual maturity (ISM) in zebra mussel using flow cytometry: interest in ecotoxicology

Abstract : The global dynamic spread of chemical contamination through the aquatic environment calls for the development of biomarkers of interest. Reproduction is a key element to be considered because it is related to the sustainability of species. Spermatogenesis is a complex process that leads to the formation of mature germ cells, whose steps and impairments need to be finely described in ecotoxicological analyses. The physiological process has been commonly described by histological analyses of gonads in different taxa. In the present paper, we describe the development of a novel technique to characterize spermatogenesis based on the analysis of the DNA content of germ cells by flow cytometry, using a DNA-intercalating agent. This new biomarker, referred to as an index of sexual maturity, proved relevant to describe the seasonal reproductive cycle of the zebra mussel, Dreissena polymorpha (Pallas, 1771), used as a sentinel species in the biomonitoring of continental waters and sensitive to highlight the reprotoxicity of carbamazepine (an anti-epileptic pharmaceutical) tested under ecosystemic conditions (mesocosms).
Type de document :
Article dans une revue
Environmental Science and Pollution Research, Springer Verlag, 2018, 25 (12), pp.11252-11263. 〈10.1007/s11356-017-9256-2〉
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-01574989
Contributeur : Agnès Bussy <>
Soumis le : jeudi 17 août 2017 - 11:16:06
Dernière modification le : jeudi 7 février 2019 - 17:44:21

Identifiants

Citation

Gabrielle Magniez, Alban Franco, , Damien Rioult, Isabelle Bonnard, et al.. Determination of a new index of sexual maturity (ISM) in zebra mussel using flow cytometry: interest in ecotoxicology. Environmental Science and Pollution Research, Springer Verlag, 2018, 25 (12), pp.11252-11263. 〈10.1007/s11356-017-9256-2〉. 〈hal-01574989〉

Partager

Métriques

Consultations de la notice

183