Tritium forms discrimination in ryegrass under constant tritium exposure: From seed germination to seedling autotrophy
Résumé
Uncertainties remain regarding the fate of atmospheric tritium after it has been assimilated in grasslands
(ryegrass) in the form of TFWT (Tissue FreeWater Tritium) or OBT (Organically Bound Tritium). One such
uncertainty relates to the tritium forms discrimination during transfer from TFWT to OBT resulting from
photosynthesis (OBTphoto), corresponding to the OBTphoto/TFWT ratio. In this study, the OBT/TFWT ratio is
determined by experiments in the laboratory using a ryegrass model and hydroponic cultures, with
constant activity of tritium in the form of tritiated water (denoted as HTO) in the “water” compartment
(liquid HTO) and “air” compartment (HTO vapour in the air). The OBTphoto/TFWT ratio and the
exchangeable OBT fraction are measured for three parts of the plant: the leaf, seed and root. Plant growth
is modelled using dehydrated biomass measurements taken over time in the laboratory and integrating
physiological functions of the plant during the first ten days after germination. The results suggest that
there is no measurable discrimination of tritium in the plant organic matter produced by photosynthesis.