Nuclear incorporation of iron during the eukaryotic cell cycle

Ian Robinson (1, 2, 3) , Yang Yang (4) , Fucai Zhang (2, 3) , Christophe Lynch (2, 3) , Mohammed Yusuf (2, 3) , Peter Cloetens (4)

1 BNL - Brookhaven National Laboratory [Upton, NY]
2 Rutherford Appleton Lab, Res Complex Harwell, Didcot OX11 0FA, Oxon, England
3 UCL, London Ctr Nanotechnol, London WC1E 6BT, England
4 ESRF - European Synchrotron Radiation Facility

Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals, presumably from DNA and associated nucleosomes, are high and relatively uniform across all the nuclei; these agree with X-ray phase contrast projection microscopy images of the same samples. Possible reasons for the Fe incorporation are discussed

Mots clés: chromosome; fluorecence; imaging; phase contrast; beamline

Domaines: Physique [physics]

Ian Robinson, Yang Yang, Fucai Zhang, Christophe Lynch, Mohammed Yusuf, et al.. Nuclear incorporation of iron during the eukaryotic cell cycle. Journal of Synchrotron Radiation, 2016, 23, pp.1490-1497. ⟨10.1107/S1600577516012807⟩. ⟨hal-01572923⟩