Controllable Microfluidic Production of Drug-Loaded PLGA Nanoparticles Using Partially Water-Miscible Mixed Solvent Microdroplets as a Precursor

Abstract : We present a versatile continuous microfluidic flow-focusing method for the production of Doxorubicin (DOX) or Tamoxifen (TAM)-loaded poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). We use a partially water-miscible solvent mixture (dimethyl sulfoxide DMSO + dichloromethane DCM) as precursor drug/polymer solution for NPs nucleation. We extrude this partially water-miscible solution into an aqueous medium and synthesized uniform PLGA NPs with higher drug loading ability and longer sustained-release ability than conventional microfluidic or batch preparation methods. The size of NPs could be precisely tuned by changing the flow rate ratios, polymer concentration, and volume ratio of DCM to DMSO (VDCM/VDMSO) in the precursor emulsion. We investigated the mechanism of the formation of NPs and the effect of VDCM/VDMSO on drug release kinetics. Our work suggests that this original, rapid, facile, efficient and low-cost method is a promising technology for high throughput NP fabrication. For the two tested drugs, one hydrophilic (Doxorubicin) the other one hydrophobic (Tamoxifen), encapsulation efficiency (EE) as high as 88% and mass loading content (LC) higher than 25% were achieved. This new process could be extended as an efficient and large scale NP production method to benefit to fields like controlled drug release and nanomedicine.
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-01567810
Contributor : Olivier Sandre <>
Submitted on : Monday, November 5, 2018 - 1:20:00 PM
Last modification on : Saturday, March 30, 2019 - 2:29:38 AM
Long-term archiving on : Wednesday, February 6, 2019 - 2:39:24 PM

File

Xu_et_al_SciRep_7_4794.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Jiang Xu, Shusheng Zhang, Anaïs Machado, Sébastien Lecommandoux, Olivier Sandre, et al.. Controllable Microfluidic Production of Drug-Loaded PLGA Nanoparticles Using Partially Water-Miscible Mixed Solvent Microdroplets as a Precursor. Scientific Reports, Nature Publishing Group, 2017, 7 (1), pp.4794. ⟨https://www.nature.com/articles/s41598-017-05184-5⟩. ⟨10.1038/s41598-017-05184-5⟩. ⟨hal-01567810⟩

Share

Metrics

Record views

43

Files downloads

100