Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols

Asma Soussou (1, 2, 3) , Ibtissem Gammoudi (1, 4) , Fabien Morote (1) , Adel Kalboussi (3) , Touria Cohen-Bouhacina (1) , Christine Grauby-Heywang (1) , Zouhair M. Baccar (2)

1 LOMA - Laboratoire Ondes et Matière d'Aquitaine
2 INRAP - Institut National de Recherche et d'Analyse Physico-Chimique
3 Laboratoire de Microélectronique et Instrumentations [Monastir]
4 Cellule de transfert NanoPhyNov

Gold screen-printed electrodes coated with thin films of layered double hydroxides containing cobalt and aluminium (Co 1.57 Al(OH) x SO 4 , shortened as CoAl) have been investigated for the design of an electrochemical tyrosinase-based biosensor used for the detection of a complex mixture of polyphenols extracted from green tea. Physicochemical analyses show that the resulted biosensor exhibits very attractive characteristics: a high sensitivity, a large dynamic range (up to 1000 ng.mL −1), and very low limits of detection (0.33 pg.mL −1 and 0.03 pg.mL −1 for oxidation and reduction, respectively).

Mots clés: Atomic force microscopy AFM; Electrochemical biosensors; Layered double hydroxide LDH; Polyphenols; Tyrosinase

Domaines: Biotechnologies; Chimie analytique

Asma Soussou, Ibtissem Gammoudi, Fabien Morote, Adel Kalboussi, Touria Cohen-Bouhacina, et al.. Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols. IEEE Sensors Journal, 2017, 17 (4), pp.4340-4348. ⟨10.1109/JSEN.2017.2709342⟩. ⟨hal-01565702⟩