Estimates and impact of lymphocyte division parameters from CFSE data using mathematical modelling.

Pauline Mazzocco 1, 2 Samuel Bernard 2, 3 Laurent Pujo-Menjouet 1, 2, 3
2 DRACULA - Multi-scale modelling of cell dynamics : application to hematopoiesis
CGPhiMC - Centre de génétique et de physiologie moléculaire et cellulaire, Inria Grenoble - Rhône-Alpes, ICJ - Institut Camille Jordan [Villeurbanne]
Abstract : Carboxyfluorescein diacetate succinimidyl ester (CFSE) labelling has been widely used to track and study cell proliferation. Here we use mathematical modelling to describe the kinetics of immune cell proliferation after an in vitro polyclonal stimulation tracked with CFSE. This approach allows us to estimate a set of key parameters, including ones related to cell death and proliferation. We develop a three-phase model that distinguishes a latency phase, accounting for non-divided cell behaviour, a resting phase and the active phase of the division process. Parameter estimates are derived from model results, and numerical simulations are then compared to the dynamics of in vitro experiments, with different biological assumptions tested. Our model allows us to compare the dynamics of CD4+ and CD8+ cells, and to highlight their kinetic differences. Finally we perform a sensitivity analysis to quantify the impact of each parameter on proliferation kinetics. Interestingly, we find that parameter sensitivity varies with time and with cell generation. Our approach can help biologists to understand cell proliferation mechanisms and to identify potential pathological division processes.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2017, 12 (6), pp.e0179768
Liste complète des métadonnées

Littérature citée [34 références]  Voir  Masquer  Télécharger
Contributeur : Samuel Bernard <>
Soumis le : jeudi 13 juillet 2017 - 13:32:17
Dernière modification le : jeudi 22 février 2018 - 13:06:08
Document(s) archivé(s) le : jeudi 25 janvier 2018 - 01:05:21


Fichiers éditeurs autorisés sur une archive ouverte


  • HAL Id : hal-01561667, version 1
  • PUBMED : 28622387


Pauline Mazzocco, Samuel Bernard, Laurent Pujo-Menjouet. Estimates and impact of lymphocyte division parameters from CFSE data using mathematical modelling.. PLoS ONE, Public Library of Science, 2017, 12 (6), pp.e0179768. 〈hal-01561667〉



Consultations de la notice


Téléchargements de fichiers