Comparative study of fully three-dimensional reconstruction algorithms for lens-free microscopy

Anthony Berdeu (1, 2) , Fabien Momey (3) , Bastien Laperrousaz (2, 4) , Thomas Bordy (1, 2) , Xavier Gidrol (2, 4) , J.-M. Dinten (1, 2) , Nathalie Picollet-D 'Hahan (2, 4) , Cédric Allier (1, 2)

1 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
2 UGA [2016-2019] - Université Grenoble Alpes [2016-2019]
3 LHC - Laboratoire Hubert Curien
4 BGE - UMR S1038 - Laboratoire de Biologie à Grande Échelle

We propose a three-dimensional (3D) imaging platform based on lens-free microscopy to perform multiangle acquisitions on 3D cell cultures embedded in extracellular matrices. Lens-free microscopy acquisitions present some inherent issues such as the lack of phase information on the sensor plane and a limited angular coverage. We developed and compared three different algorithms based on the Fourier diffraction theorem to obtain fully 3D reconstructions. These algorithms present an increasing complexity associated with a better reconstruction quality. Two of them are based on a regularized inverse problem approach. To compare the reconstruction methods in terms of artefact reduction, signal-to-noise ratio, and computation time, we tested them on two experimental datasets: an endothelial cell culture and a prostate cell culture grown in a 3D extracellular matrix with large reconstructed volumes up to ∼5  mm3∼5  mm3 with a resolution sufficient to resolve isolated single cells. The lens-free reconstructions compare well with standard microscopy.

Domaines: Traitement du signal et de l'image [eess.SP]; Imagerie

Anthony Berdeu, Fabien Momey, Bastien Laperrousaz, Thomas Bordy, Xavier Gidrol, et al.. Comparative study of fully three-dimensional reconstruction algorithms for lens-free microscopy. Applied optics, 2017, 56 (13), pp.3939-3951. ⟨10.1364/AO.56.003939⟩. ⟨hal-01561175⟩