NanoHPLC with parallel electrospray and inductively coupled plasma MS detection for biomolecules analysis.

Pierre Giusti (1) , Dirk Schaumlöffel (2) , Ryszard Lobinski (3) , Joanna Szpunar (2)

1 TOTAL Refining and Chemical
2 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux
3 LCABIE - Laboratoire de Chimie Analytique Bio-Inorganique et Environnement

The inductively coupled plasma mass spectrometer (ICP-MS) is an element and isotope specific technique which provides detection limits at the femtogram level regardless of the mol. structure of the analyte and its coelution with matrix components and interfering compds. These features make ICP-MS an ideal complementary detection technique to electrospray MS/MS in HPLC of metal complexes or biomols. contg. in their structure a heteroatom, such as, for example, sulfur, phosphorous, selenium or iodine. This article discusses two exptl. setups for interfacing nanoHPLC to ICP-MS and highlights potential added value of ICP-MS for quant. peptides mapping and proteins characterization. [on SciFinder(R)]

Mots clés: nano HPLC mass spectrometry ESI ICP isotope diln selenopeptide

Domaines: Chimie analytique

Pierre Giusti, Dirk Schaumlöffel, Ryszard Lobinski, Joanna Szpunar. NanoHPLC with parallel electrospray and inductively coupled plasma MS detection for biomolecules analysis.. Spectra Analyse, 2006, 35 (Copyright (C) 2017 American Chemical Society (ACS). All Rights Reserved.), pp.38--43. ⟨hal-01560771⟩