Investigation of the aluminium binding in Al(III)-treated neuroblastoma cells

K. Polłéc-Pawlak , P. Zambenedetti , Joanna Szpunar (1) , Ryszard Lobinski (2) , P. Zatta

1 LCABIE - Laboratoire de Chimie Analytique Bio-Inorganique et Environnement
2 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Different hyphenated techniques have been employed for the first time for a comparative study of control and metal-exposed cell lines, one of the important research areas in the field of metallomics. Speciation of aluminium (an element implicated in a variety of neurological disorders) was investigated in neuroblastoma cells exposed to aluminium lactate by size-exclusion high-performance liquid chromatography-inductively coupled plasma mass spectrometry (SE-HPLC-ICP-MS) and capillary electrophoresis (CE-ICP-MS). Whereas in control cells the most intense Al compound co-eluted with the Al-transferrin complex, in size-exclusion chromatography, in exposed cells a low molecular weight bioligand was synthesized. The latter bound all the Al(III) metabolised, by exposed cells in the form of a negatively-charged complex. This complex turned out to be more stable than the protein complex of Al(III) in control cells. The isolated low molecular weight Al species did not produce a signal in electrospray mass spectrometry (ES-MS) and its behaviour investigated by size-exclusion, reversed-phase HPLC and CE excluded its being hydroxide, citrate, phosphate or residual lactate from the culture medium. © The Royal Society of Chemistry 2004.

Domaines: Chimie analytique

K. Polłéc-Pawlak, P. Zambenedetti, Joanna Szpunar, Ryszard Lobinski, P. Zatta. Investigation of the aluminium binding in Al(III)-treated neuroblastoma cells. Journal of Analytical Atomic Spectrometry, 2004, 19 (1), pp.41--45. ⟨10.1039/b307767p⟩. ⟨hal-01560731⟩