Continuous hydrolysis of /3-casein in a membrane reactor: Preparation of bioactive peptide

Said Bouhallab (1) , Daniel Mollé (1) , Joelle Léonil (1)

1 STLO - Science et Technologie du Lait et de l'Oeuf

Continuous Stirred Tank Membrane Reactor was used to investigate the continuous and selective extraction of a bioactive peptide B-CN (193-209) from bovine B-casein/chymosin hydrolysate. It was shown that the feasibility of the process depends on the nature and the area of ultrafiltration membrane used. With an inorganic (carbon-zirconia) membrane, high retention of the peptide constitutes a limit to the operation. However, when the reactor was equipped with a cellulosic type membrane, satisfactory transmission of the peptide was obtained. As shown by RP-HPLC and mass spectrometry analysis, only /3-CN (193-209) permeates through the membrane.

Mots clés: hydrolyse

Mots clés: membrane; beta caséine; peptide bioactif; ultrafiltration

Domaines: Alimentation et Nutrition; Ingénierie des aliments

Said Bouhallab, Daniel Mollé, Joelle Léonil. Continuous hydrolysis of /3-casein in a membrane reactor: Preparation of bioactive peptide. Biotechnology Letters, 1993, 15 (7), pp.697-702. ⟨hal-01559699⟩