First landscape of binding to chromosomes for a domesticated mariner transposase in the human genome: diversity of genomic targets of SETMAR isoforms in two colorectal cell lines

Abstract : Setmar is a 3-exons gene coding a SET domain fused to a Hsmar1 transposase. Its different transcripts theoretically encode 8 isoforms with SET moieties differently spliced. In vitro, the largest isoform binds specifically to Hsmar1 DNA ends and with no specificity to DNA when it is associated with hPso4. In colon cell lines, we found they bind specifically to two chromosomal targets depending probably on the isoform, Hsmar1 ends and sites with no conserved motifs. We also discovered that the isoforms profile was different between cell lines and patient tissues, suggesting the isoforms encoded by this gene in healthy cells and their functions are currently not investigated.
Keywords : MADE1 Hsmar1
Document type :
Preprints, Working Papers, ...
Complete list of metadatas

Cited literature [4 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01558001
Contributor : Catherine Belleannée <>
Submitted on : Thursday, July 6, 2017 - 7:14:11 PM
Last modification on : Monday, December 23, 2019 - 11:08:12 AM
Long-term archiving on: Wednesday, January 24, 2018 - 7:32:02 AM

File

115030.full.pdf
Files produced by the author(s)

Identifiers

Citation

Aymeric Antoine-Lorquin, Ahmed Arnaoty, Sassan Asgari, Martine Batailler, Linda Beauclair, et al.. First landscape of binding to chromosomes for a domesticated mariner transposase in the human genome: diversity of genomic targets of SETMAR isoforms in two colorectal cell lines. 2017. ⟨hal-01558001⟩

Share

Metrics

Record views

517

Files downloads

344