HPLC-PAD-APCI assay of phenylpropanoids in cereals

A.C. Bily , A.J. Burt , A.-I. Ramputh , J. Livesey , Catherine Regnault-Roger (1) , B.R. Philogène , J.T. Arnason

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

A new, rapid HPLC-PAD-APCI/MS assay has been developed in order to measure accurately the amount of p-coumaric, E- and Z-ferulic acid and the dehydrodimers of ferulic acid in cereal grain. In the positive ionisation mode, MS patterns gave additional information for the identification of the dimers. The time required and the quantities of solvents employed in the developed analytical method are much lower than those involved in previously available assays of these compounds, thus making the method suitable for the screening of cereal geno-types. Application of the method to accessions of maize, wheat and sorghum showed that E-ferulic was the most abundant phenylpropanoid, whilst the major dimer was 8-O-4′ dehydrodimer of ferulic acid followed by the 5-5′ and then the 8-5′ forms. Maize grains, especially of the Mexican landraces, contained the highest levels of these dimers. Copyright © 2004 John Wiley & Sons, Ltd.

Domaines: Biologie végétale

A.C. Bily, A.J. Burt, A.-I. Ramputh, J. Livesey, Catherine Regnault-Roger, et al.. HPLC-PAD-APCI assay of phenylpropanoids in cereals. Phytochemical Analysis, 2004, 15 (1), pp.9-15. ⟨10.1002/pca.735⟩. ⟨hal-01557880⟩