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Journal articles
O2-independent formation of the inactive states of NiFe hydrogenase
Abbas Abou Hamdan
1
Benedicte Burlat
1
Oscar Gutierrez-Sanz
2
Pierre-Pol Liebgott
1
Carole Baffert
1
Antonio L de Lacey
2
Marc Rousset
1
Bruno Guigliarelli
1
Christophe Léger
1
Sebastien Dementin
1
Abbas Abou Hamdan 1
Author
Benedicte Burlat 1
Mail :
Author
Oscar Gutierrez-Sanz 2
Author
Pierre-Pol Liebgott 1
Mail :
Author IdHAL : pierre-pol-liebgott ORCID : https://orcid.org/0000-0002-2559-1738
Carole Baffert 1
Author
Antonio L de Lacey 2
Author
Marc Rousset 1
Author
Bruno Guigliarelli 1
Author
Christophe Léger 1
Mail :
Author IdHAL : christophe-leger ResearcherId : http://www.researcherid.com/rid/A-4115-2008 ORCID : https://orcid.org/0000-0002-8871-6059
Sebastien Dementin 1
Mail :
Author
1
BIP - Bioénergétique et Ingénierie des Protéines (CNRS - Université d’ Aix-Marseille
31, chemin Joseph Aiguier
13402 MARSEILLE Cedex 20 FRANCE
Tél : +33 (0)4 91 16 45 50 - France)
- AMU - Aix Marseille Université : UMR7281 (Aix-Marseille Université Jardins du Pharo 58 Boulevard Charles Livon 13284 Marseille cedex 7 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7281 (France)
2
CSIC - Centro de Investigaciones Biológicas (Ramiro de Maeztu Madrid - Spain)
- CSIC - Consejo Superior de Investigaciones Científicas [Spain] (Serrano 117, 28006 Madrid - Spain)
Abstract : We studied the mechanism of aerobic inactivation of Desulfovibrio fructosovorans nickel-iron (NiFe) hydrogenase by quantitatively examining the results of electrochemistry, EPR and FTIR experiments. They suggest that, contrary to the commonly accepted mechanism, the attacking O2 is not incorporated as an active site ligand but, rather, acts as an electron acceptor. Our findings offer new ways toward the understanding of O2 inactivation and O2 tolerance in NiFe hydrogenases.
Document type :
Journal articles
Complete list of metadatas
https://hal-amu.archives-ouvertes.fr/hal-01556905
Contributor : Laure Azzopardi <>
Submitted on : Wednesday, July 5, 2017 - 4:29:54 PM
Last modification on : Monday, September 23, 2019 - 5:56:02 PM
Contributor : Laure Azzopardi <>
Submitted on : Wednesday, July 5, 2017 - 4:29:54 PM
Last modification on : Monday, September 23, 2019 - 5:56:02 PM
Identifiers
- HAL Id : hal-01556905, version 1
- DOI : 10.1038/nchembio.1110
Citation
Abbas Abou Hamdan, Benedicte Burlat, Oscar Gutierrez-Sanz, Pierre-Pol Liebgott, Carole Baffert, et al.. O2-independent formation of the inactive states of NiFe hydrogenase. Nature Chemical Biology, Nature Publishing Group, 2013, 9 (1), pp.15 - 17. ⟨10.1038/nchembio.1110⟩. ⟨hal-01556905⟩
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