Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte

Piia Liigand (1) , Karl Kaupmees (1) , Kristjan Haav (1) , Jaanus Liigand (1) , Ivo Leito (1) , Marion Girod (2) , Rodolphe Antoine (3) , Anneli Kruve (1, 4)

1 Institute of Chemistry
2 ISA-ANABIO-MS - Analyse biomoléculaire par spectrométrie de masse - Biological Analysis by Mass Spectrometry
3 ILM - Institut Lumière Matière [Villeurbanne]
4 Technion Israel Inst Technol

For the first time, the electrospray ionization efficiency (IE) scales in positive and negative mode are united into a single system enabling,direct comparison of IE values across ionization modes. This is made possible by the use of a reference Compound that ionizes to a similar extent in both positive, and negative modes. Thus, choosing the optimal (i.e., most sensitive) ionization conditions for a given set of analytes is enabled. Ionizatiori efficiencies of 33 compounds ionizing in both modes demonstrate that, contrary to general practice, negative mode allows, better sensitivity for 46% of such compounds Whereas the positive mode is preferred for only 18%, and for 36%, the results for both modes are comparable.

Domaines: Chimie analytique

Piia Liigand, Karl Kaupmees, Kristjan Haav, Jaanus Liigand, Ivo Leito, et al.. Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte. Analytical Chemistry, 2017, 89 (11), pp.5665-5668. ⟨10.1021/acs.analchem.7b00096⟩. ⟨hal-01546359⟩