CD4(+) T cell surface alpha enolase is lower in older adults

Abstract : To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4(+) T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n = 9, 20-25 years) and older (n = 10; 50-70 years) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences <0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4(+) T cell surface alpha-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n = 22) and qPCR with significantly lower expression of cellular alpha-enolase mRNA and protein compared to young adult CD4(+) T cells (p < 0.05). In an independent age-matched case-control study, lower CD4(+) T cell surface et-enolase expression was observed in age-matched patients with cardiovascular disease (p < 0.05). An immune-modulatory role has been proposed for surface alpha-enolase and our findings of decreased expression suggest that deficits in surface alpha-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.
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Stuart J. Bennett, Edyta M. Augustyniak, Christopher R. Dunston, Richard A. Brown, Eduard Shantsila, et al.. CD4(+) T cell surface alpha enolase is lower in older adults. Mechanisms of Ageing and Development, Elsevier, 2015, 152, pp.56-62. ⟨10.1016/j.mad.2015.09.005⟩. ⟨hal-01544058⟩



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