Gammarus fossarum as a sensitive tool to reveal residual toxicity of treated wastewater effluents

Adriana Wigh (1, 2) , Olivier Geffard (3) , Khedidja Abbaci (3) , Adeline François (3) , Patrice Noury (3) , Alexandre Bergé (4) , Emmanuelle Vulliet (4) , Bruno Domenjoud (5) , Adriana Gonzalez-Ospina (5) , Sylvie Bony (1, 2) , Alain Devaux (1, 2)

1 IAPHY - Équipe 5 - Impact des Aménagements et des Polluants sur les HYdrosystèmes
2 LEHNA - Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés
3 IRSTEA - Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture
4 ISA - Institut des Sciences Analytiques
5 SUEZ Traitement de l'eau

Wastewater treatment plants (WWTPs) are one of the main sources of freshwater pollution eventually resulting in adverse effects in aquatic organisms. Treated effluents can contain many micropollutants at concentrations often below the limit of chemical quantification. On a regulatory basis, WWTP effluents have to be non-toxic to the aquatic environment, wherefore not only chemical abatement but also ecotoxicological evaluation through relevant bioassays is required. Standardized bioassays currently used are often not sensitive enough to reveal a residual toxicity in treated effluents. Therefore, attention must be paid to the development of better-adapted approaches implementing more sensitive organisms and relevant endpoints. In this study, the toxicity of two differently treated effluents (activated sludge treated effluents with and without ozonation) towards the ecologically relevant species Gammarus fossarum was evaluated. Organism fitness traits such as reproduction and sperm DNA integrity were followed in exposed organisms. In complement, enzymatic biomarkers were measured indicating the presence of neurotoxic compounds (acetylcholinesterase activity), the presence of pathogens likely to increase the toxic effects of chemical compounds (phenol-oxidase activity), and the presence of toxic compounds inducing detoxification mechanisms (glutathione-S-transferase activity). Enzymatic activities were not modified, but significant sub-lethal effects were observed in exposed organisms. In both effluents, females showed a retarded molt cycle, a reduced fecundity and fertility, and > 90% of developed embryos exhibited developmental malformations. In addition, a slight but significant genotoxic effect was measured in gammarid sperm. In a whole, no difference in toxicity was found between both effluents. Coupling reproduction impairment and genotoxicity assessment in Gammarus fossarum seems to be a valuable and sensitive tool to reveal residual toxicity in effluents containing a mixture of micropollutants at very low concentrations. Finally, a direct relationship between the observed toxic responses and the quantified micropollutant concentrations could not be evidenced.

Mots clés: Reprotoxicity; Genotoxicity; Micropollutant; Ozonation

Domaines: Chimie analytique; Sciences de l'environnement

Adriana Wigh, Olivier Geffard, Khedidja Abbaci, Adeline François, Patrice Noury, et al.. Gammarus fossarum as a sensitive tool to reveal residual toxicity of treated wastewater effluents. Science of the Total Environment, 2017, 584, pp.1012-1021. ⟨10.1016/j.scitotenv.2017.01.154⟩. ⟨hal-01539979⟩