Transcriptomic analysis of mouse limb tendon cells during development

Abstract : The molecular signals driving tendon development are not fully identified. We have undertaken a transcriptome analysis of mouse limb tendon cells that were isolated at different stages of development based on scleraxis ( Scx) expression. Microarray comparisons allowed us to establish a list of genes regulated in tendon cells during mouse limb development. Bioinformatics analysis of the tendon transcriptome showed that the two most strongly modified signalling pathways were TGF-beta and MAPK. TGF-beta/SMAD2/3 gain- and loss-of-function experiments in mouse limb explants and mesenchymal stem cells showed that TGF-beta signalling was sufficient and required via SMAD2/3 to drive mouse mesodermal stem cells towards the tendon lineage ex vivo and in vitro. TGF-beta was also sufficient for tendon gene expression in late limb explants during tendon differentiation. FGF does not have a tenogenic effect and the inhibition of the ERK MAPK signalling pathway was sufficient to activate Scx in mouse limb mesodermal progenitors and mesenchymal stem cells.
Type de document :
Article dans une revue
Development (Cambridge, England), Company of Biologists, 2014, 141 (19), pp.3683-3696. 〈10.1242/dev.108654〉
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-01538325
Contributeur : Charlotte Mazalérat <>
Soumis le : mardi 13 juin 2017 - 13:59:16
Dernière modification le : vendredi 31 août 2018 - 09:18:16

Lien texte intégral

Identifiants

Collections

Citation

Emmanuelle Havis, Marie-Ange Bonnin, Isabel Olivera-Martinez, Nicolas Nazaret, Mathilde Ruggiu, et al.. Transcriptomic analysis of mouse limb tendon cells during development. Development (Cambridge, England), Company of Biologists, 2014, 141 (19), pp.3683-3696. 〈10.1242/dev.108654〉. 〈hal-01538325〉

Partager

Métriques

Consultations de la notice

87