Identification of ageing biomarkers in human dermis biopsies by thermal analysis (DSC) combined with Fourier transform infrared spectroscopy (FTIR/ATR)

Abstract : Background/purpose : The purpose of this clinical study was to identify suitable biomarkers for a better understanding of the molecular and organizational changes in human dermis during intrinsic and extrinsic ageing. Methods : Sun-exposed and non-exposed skin biopsies were collected from twenty-eight women devised in two groups (20-30 and ≥60 years old). The hydric organization and thermal transitions were determined by Differential Scanning Calorimetry (DSC). Fourier Transform Infrared spectroscopy (FTIR) was used to identify the absorption bands of the dermis and to quantify the different absorbance ratio. Results : The amounts of total, freezable and unfreezable water were determined. A significant increasing amount of freezable water is evidenced in sun-exposed area skin of aged group compared with young group (P=.0126). Another significant effect of extrinsic ageing (P=.0489) is the drastic decrease of fibrillary collagen, the main protein component of dermis. The only significant effect of intrinsic ageing (P=.0184) is an increase of the heat-stable fraction of collagens in dermis. Conclusion : DSC and FTIR are well-suited techniques to characterize human skin, giving accurate results with a high reproducibility. The combination of these techniques is useful for a better understanding of human skin modifications with intrinsic and extrinsic ageing.
Document type :
Journal articles
Complete list of metadatas

Cited literature [8 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01538132
Contributor : Open Archive Toulouse Archive Ouverte (oatao) <>
Submitted on : Tuesday, June 13, 2017 - 12:10:59 PM
Last modification on : Friday, June 14, 2019 - 6:31:15 PM
Long-term archiving on : Tuesday, December 12, 2017 - 1:09:03 PM

File

tang_17892.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Rong Tang, Valérie Samouillan, Jany Dandurand, Colette Lacabanne, Marie-Hélène Lacoste-Ferré, et al.. Identification of ageing biomarkers in human dermis biopsies by thermal analysis (DSC) combined with Fourier transform infrared spectroscopy (FTIR/ATR). Skin Research and Technology, Wiley, 2017, pp.1-8. ⟨10.1111/srt.12373⟩. ⟨hal-01538132⟩

Share

Metrics

Record views

79

Files downloads

101