Selective and Cooperative Ligand Binding to Antiparallel Human Telomeric DNA G-Quadruplexes

Adrien Marchand (1) , Dominika Strzelecka (1) , Valérie Gabelica (1)

1 ARNA - Acides Nucléiques : Régulations Naturelle et Artificielle

The quest for ligands that bind specifically to particular G-quadruplex nucleic acid structures is particularly important to conceive molecules with specific effects on gene expression or telomere maintenance, or conceive structure-specific molecular probes. Here, using electrospray mass spectrometry in native conditions, we reveal a highly cooperative and selective 2:1 binding of Cu(II)-tolylterpyridine complexes to human telomeric G-quadruplexes. Circular dichroism and comparisons of affinities for different sequences reveal a marked preference for antiparallel structures with diagonal loops and/or wide-medium-narrow-medium groove width order. The cooperativity is attributed to conformational changes in the polymorphic telomeric G-quadruplex sequences, which convert preferably to an antiparallel, 3-quartet topology upon binding two ligands.

Mots clés: Mass spectrometry MS; Quadruplex DNA; Ligand binding modes

Domaines: Chimie analytique; Chimie théorique et/ou physique; Biophysique; Biologie structurale [q-bio.BM]; Biochimie [q-bio.BM]

Adrien Marchand, Dominika Strzelecka, Valérie Gabelica. Selective and Cooperative Ligand Binding to Antiparallel Human Telomeric DNA G-Quadruplexes. Chemistry - A European Journal, 2016, 22 (28), pp.9551-9555. ⟨10.1002/chem.201601937⟩. ⟨hal-01537094⟩