Thermal and vibrational characterization of human skin

Abstract : For a better understanding of the molecular and organizational changes in human dermis, biophysical methods were tested. The aim of this study was to find suitable and reproducible biomarkers for further clinical studies on intrinsic and extrinsic aging of dermis. Thermoporometry, hydric organization and thermal transitions of fresh and frozen skins were determined by differential scanning calorimetry (DSC). Fourier transform infrared spectroscopy (FTIR) was used to identify the absorption bands of the dermis especially in the 1800–1000 cm−1 zone and to discriminate between the different secondary structures of proteins. A widening of the pore size distribution is evidenced with freezing, but there is no significant difference between the hydric organization and the endothermic collagen denaturation of fresh and frozen skins. The global FTIR spectra and the second derivative spectra in the scanned zone are also identical in fresh and frozen dermis, validating the storage protocol. DSC and FTIR are well-suited techniques to characterize human skin, giving accurate results with high reproducibility. The acquisition of thermal and vibrational biomarkers of the skin at the mesoscale and nanoscale contributes to its better knowledge and is promising for further studies on skin aging.
Document type :
Journal articles
Complete list of metadatas

Cited literature [94 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01536722
Contributor : Open Archive Toulouse Archive Ouverte (oatao) <>
Submitted on : Monday, June 12, 2017 - 11:04:23 AM
Last modification on : Friday, January 10, 2020 - 9:08:23 PM
Long-term archiving on: Thursday, September 14, 2017 - 12:28:06 PM

File

tang_17887.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Rong Tang, Valérie Samouillan, Jany Dandurand, Colette Lacabanne, Florence Nadal-Wollbold, et al.. Thermal and vibrational characterization of human skin. Journal of Thermal Analysis and Calorimetry, Springer Verlag, 2017, vol. 127 (n° 2), pp. 1143-1154. ⟨10.1007/s10973-016-5384-z⟩. ⟨hal-01536722⟩

Share

Metrics

Record views

122

Files downloads

139