Sperm quality parameters and reproductive efficiency in Muscovy duck (Cairina moschata)

Yi-Chen Chen (1, 2) , Hsiu-Chou Liu (3) , Liang-Yuan Wei (3) , Jeng-Fang Huang (3) , Chai-Ching Lin (1) , Elisabeth Blesbois (4) , Ming-Cheng Chen (1)

1 Department of Biotechnology and Animal Science
2 Institute of Biotechnology
3 Ilan Branch, Livestock Research Institute
4 PRC - Physiologie de la reproduction et des comportements [Nouzilly]

The in vitro sperm quality parameters (motility, M; viability, V; normal morphology, NM; plasma membrane integrity, PMI; mitochondrial function, MF) in Muscovy drakes (Cairina moschata) were evaluated by using microscopy and flow cytometry, the correlation among sperm quality parameters and results of artificial insemination was also assessed in present study. M, V and NM were detected by phase contrast microscopy assisted with eosin-nigrosin staining, and PMI and MF were detected by using flow cytometry within appropriate fluorescence staining (SYBR-14/PI and R123/PI, respectively). Fertility (F), early embryonic mortality (EEM) and the survival embryo rate (SER) were assessed after the artificial insemination of Muscovy or Kaiya duck (Anas platyrhynchos) females. Sperm PMI and MF, the parameters detected by flow cytometry were positively correlated with sperm M, V, and NM, those were detected by phase contrast microscopy (P < 0.05). Sperm V and PMI were negatively correlated with the percentage of early embryo mortality of Muscovy duck fertile eggs (P < 0.05). The results of the present study showed the relationships among the AI results and the sperm quality parameters detected by microscopy as well as flow cytometry. In conclusion, flow cytometry assisted with microscopy can be an effective tool to evaluate in vitro sperm quality and may contribute to predict the reproductive performances of individual Muscovy drakes, which helps to improve duck production efficiency.

Mots clés: artificial insemination; sperm evaluation; Muscovy duck; duck

Mots clés: cairina moschata; canard; insémination artificielle; qualité du sperme; reproduction mâle; motilité du sperme

Domaines: Biologie de la reproduction; Autre [q-bio.OT]

Yi-Chen Chen, Hsiu-Chou Liu, Liang-Yuan Wei, Jeng-Fang Huang, Chai-Ching Lin, et al.. Sperm quality parameters and reproductive efficiency in Muscovy duck (Cairina moschata). The Journal of Poultry Science, 2016, 53 (3), pp.223-232. ⟨10.2141/jpsa.0150162⟩. ⟨hal-01529463⟩