Identification of misexpressed genetic elements in hybrids between Drosophila-related species

Hélène Lopez-Maestre 1, 2 Elias A. G. Carnelossi 3 Vincent Lacroix 4, 2 Nelly Burlet 1 Bruno Mugat 5 Séverine Chambeyron 5 Claudia M. A. Carareto 3 Cristina Vieira 6, 1, 2
1 Eléments transposables, évolution, populations
GINSENG - Département génétique, interactions et évolution des génomes [LBBE]
2 ERABLE - Equipe de recherche européenne en algorithmique et biologie formelle et expérimentale
Inria Grenoble - Rhône-Alpes
3 UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho [São José do Rio Preto]
4 Baobab
PEGASE - Département PEGASE [LBBE]
5 IGH - Institut de génétique humaine
6 IUF - Institut Universitaire de France
Abstract : Crosses between close species can lead to genomic disorders, often considered to be the cause of hybrid incompatibility, one of the initial steps in the speciation process. How these incompatibilities are established and what are their causes remain unclear. To understand the initiation of hybrid incompatibility, we performed reciprocal crosses between two species of Drosophila (D. mojavensis and D. arizonae) that diverged less than 1 Mya. We performed a genome-wide transcriptomic analysis on ovaries from parental lines and on hybrids from reciprocal crosses. Using an innovative procedure of co-assembling transcriptomes, we show that parental lines differ in the expression of their genes and transposable elements. Reciprocal hybrids presented specific gene categories and few transposable element families misexpressed relative to the parental lines. Because TEs are mainly silenced by piwi-interacting RNAs (piRNAs), we hypothesize that in hybrids the deregulation of specific TE families is due to the absence of such small RNAs. Small RNA sequencing confirmed our hypothesis and we therefore propose that TEs can indeed be major players of genome differentiation and be implicated in the first steps of genomic incompatibilities through small RNA regulation.
Document type :
Journal articles
Complete list of metadatas

Cited literature [63 references]  Display  Hide  Download
https://hal.archives-ouvertes.fr/hal-01524879
Contributor : Catherine Larose <>
Submitted on : Monday, May 29, 2017 - 11:29:23 AM
Last modification on : Tuesday, November 5, 2019 - 2:52:10 PM

File

srep40618 (1).pdf
Publisher files allowed on an open archive

Identifiers

Collections

INRIA | BIOENVIS | BS | UNIV-MONTPELLIER | UNIV-LYON1

Citation

Hélène Lopez-Maestre, Elias A. G. Carnelossi, Vincent Lacroix, Nelly Burlet, Bruno Mugat, et al.. Identification of misexpressed genetic elements in hybrids between Drosophila-related species. Scientific Reports, Nature Publishing Group, 2017, 7, pp.40618. ⟨10.1038/srep40618⟩. ⟨hal-01524879v2⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

349

Files downloads

150

Contact

CCSD