Association between Polymorphisms in the Pfmdr6 Gene and Ex Vivo Susceptibility to Quinine in Plasmodium falciparum Parasites from Dakar, Senegal

Mathieu Gendrot (1, 2) , Silman Diawara (3) , Marylin Madamet (2) , Mame Bou Kounta (3) , Sebastien Briolant (2) , Khalifa Ababacar Wade (3) , Mansour Fall (4) , Nicolas Benoit (5, 6, 7) , Aminata Nakoulima (8) , Remy Amalvict (2) , Yaya Diemé (9) , Bécaye Fall (9) , Boubacar Wade (3) , Bakary Diatta (4) , Bruno Pradines (2)

1 IRBA - Institut de Recherche Biomédicale des Armées [Antenne Marseille]
2 URMITE - Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes
3 Hôpital Principal de Dakar
4 Service de Réanimation Médicale
5 IP-TPT - Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques
6 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
7 Centre National de Référence du Paludisme
8 Service de pédiatrie
9 Laboratoire d'étude de la chimiosensibilité du paludisme

Polymorphisms and the overexpression of transporter genes, especially of the ATP-binding cassette superfamily, have been involved in antimalarial drug resistance. The objective of this study was to use 77 Senegalese Plasmodium falciparum isolates to evaluate the association between the number of Asn residues in the polymorphic microsatellite region of the Plasmodium falciparum multidrug resistance 6 gene (Pfmdr6) and the ex vivo susceptibility to antimalarials. A significant association was observed between the presence of 7 or 9 Asn repeats and reduced susceptibility to quinine.

Mots clés: antimalarial drug; Plasmodium falciparum; malaria; in vitro; resistance; molecular marker

Domaines: Maladies infectieuses

Mathieu Gendrot, Silman Diawara, Marylin Madamet, Mame Bou Kounta, Sebastien Briolant, et al.. Association between Polymorphisms in the Pfmdr6 Gene and Ex Vivo Susceptibility to Quinine in Plasmodium falciparum Parasites from Dakar, Senegal. Antimicrobial Agents and Chemotherapy, 2017, 61 (3), ⟨10.1128/AAC.01183-16⟩. ⟨hal-01521240⟩