Hyperpolarization of Nitrogen-15 Nuclei by Cross Polarization and Dissolution Dynamic Nuclear Polarization

Jonas Milani (1, 2) , Basile Vuichoud (1, 2) , Aurélien Bornet (2) , Roberto Melzi (3) , Sami Jannin (2) , Geoffrey Bodenhausen (4, 5)

1 ISA - Hyperpolarized Magnetic Resonance
2 ISIC - Institut des Sciences et Ingénierie Chimiques
3 Bruker BioSpin
4 UPMC - Université Pierre et Marie Curie - Paris 6
5 LBM UMR 7203 - Laboratoire des biomolécules

Dynamic Nuclear Polarization (DNP) is often achieved by the direct transfer of polarization from electrons to nuclei such as C-13, induced by microwave saturation of the wings of narrow EPR lines of radicals like trityl. In the indirect approach on the other hand, DNP is used to transfer the polarization from the electrons of radicals such as nitroxides that have broad EPR lines to nuclear spins I = H-1, followed by cross-polarization (CP) from I = H-1 to S = C-13 or other nuclei with low gyromagnetic ratios. This approach is particularly attractive for S = N-15, since direct DNP yields modest polarizations P(N-15) < 4% with build-up times that can be as long as tau DNP(N-15) > 2 h. In this paper, we show that CP from H-1 to N-15 at 1.2 K can yield P(N-15) = 25% with iota CP-DNP(N-15) = 10-15 min. After rapid dissolution and transfer to a solution-state NMR spectrometer, a polarization P(N-15) = 20% was observed at 300 K. The longitudinal relaxation times in solution can be as long as T-1(N-15) > 800 s in favorable cases.

Domaines: Chimie-Physique [physics.chem-ph]; Chimie analytique

Jonas Milani, Basile Vuichoud, Aurélien Bornet, Roberto Melzi, Sami Jannin, et al.. Hyperpolarization of Nitrogen-15 Nuclei by Cross Polarization and Dissolution Dynamic Nuclear Polarization. Review of Scientific Instruments, 2017, 88 (1), pp.015109. ⟨10.1063/1.4973777⟩. ⟨hal-02320893⟩